modified pet28a vector Search Results


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Millipore expression vector p28
Cloning details
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Cloning details
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Merck & Co pet-28a vector
Cloning details
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Image Search Results


Cloning details

Journal: Acta Crystallographica. Section F, Structural Biology Communications

Article Title: Crystallographic analysis of RsmA, a ribosomal RNA small subunit methyltransferase A from Staphylococcus aureus

doi: 10.1107/S2053230X15011279

Figure Lengend Snippet: Cloning details

Article Snippet: The PCR fragment was digested by the BamHI and XhoI restriction endo­nucleases and then inserted into expression vector p28 (Novagen, a modification of pET-28a) to give a hexahistidine tag (MGHHHHHH) at the N-terminus of the recombinant RsmA.

Techniques: Clone Assay, Plasmid Preparation, Modification, Expressing, Sequencing, Construct, Produced